| 出品公司: | Promega |
|---|---|
| 载体名称: | pNL2.1[Nluc/Hygro] |
| 质粒类型: | 荧光素酶载体 |
| 高拷贝/低拷贝: | 高拷贝 |
| 克隆方法: | -- |
| 启动子: | -- |
| 载体大小: | 4709 bp |
| 5' 测序引物及序列: | -- |
| 3' 测序引物及序列: | -- |
| 载体标签: | -- |
| 载体抗性: | 氨苄青霉素 |
| 筛选标记: | 潮霉素 |
| 克隆菌株: | -- |
| 宿主细胞（系）: | -- |
| 备注: | 无启动子载体，用于通过NanoLuc®荧光素酶测定测定启动子和增强子序列的活性。 |
| 产品目录号: | -- |
| 稳定性: | -- |
| 组成型/诱导型: | -- |
| 病毒/非病毒: | -- |
买家导航
pNL2.1[Nluc/Hygro]载体质粒基本信息
pNL2.1[Nluc/Hygro]质粒图谱载体图谱和pNL2.1[Nluc/Hygro]载体序列质粒序列多克隆位点信息
![pNL2.1[Nluc-Hygro]载体图谱](/uploads/载体图谱/pNL2.1[Nluc-Hygro]载体图谱.png)
pNL2.1[Nluc/Hygro]质粒载体简介
pNL2.1[Nluc/Hygro]质粒序列载体序列
LOCUS pNL2.1[Nluc Hygro] 4709 bp ds-DNA circular SYN 26-JUL-2013
DEFINITION Promoterless vector for measuring the activity of promoter and
enhancer sequences with a NanoLuc(R) luciferase assay.
ACCESSION JQ513372
VERSION .
KEYWORDS pNL2.1[Nluc Hygro]
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 4709)
AUTHORS Promega
TITLE Direct Submission
JOURNAL Exported Wednesday, February 26, 2020 from SnapGene Viewer 3.1.4
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..4709
/organism="synthetic DNA construct"
/lab_host="Mammalian Cells"
/mol_type="other DNA"
misc_feature 1..70
/note="MCS"
/note="multiple cloning site"
CDS 100..615
/codon_start=1
/product="NanoLuc(R) luciferase"
/note="Nluc"
/note="human codon-optimized"
/translation="MVFTLEDFVGDWRQTAGYNLDQVLEQGGVSSLFQNLGVSVTPIQR
IVLSGENGLKIDIHVIIPYEGLSGDQMGQIEKIFKVVYPVDDHHFKVILHYGTLVIDGV
TPNMIDYFGRPYEGIAVFDGKKITVTGTLWNGNKIIDERLINPDGSLLFRVTINGVTGW
RLCERILA"
polyA_signal 664..785
/note="SV40 poly(A) signal"
/note="SV40 polyadenylation signal"
promoter 979..1336
/note="SV40 promoter"
/note="SV40 enhancer and early promoter"
rep_origin 1187..1322
/note="SV40 ori"
/note="SV40 origin of replication"
CDS 1367..2404
/codon_start=1
/product="hygromycin B phosphotransferase"
/note="HygR"
/note="confers resistance to hygromycin"
/translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
EVGRV"
polyA_signal 2428..2476
/note="synthetic polyadenylation signal"
rep_origin complement(2803..3391)
/direction=LEFT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(3591..4451)
/codon_start=1
/product="beta-lactamase"
/note="AmpR"
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
polyA_signal 4556..4604
/note="synthetic polyadenylation signal"
misc_feature 4618..4709
/note="pause site"
/note="RNA polymerase II transcriptional pause signal from
the human alpha-2 globin gene"
ORIGIN
1 ggcctaactg gccggtacct gagctcgcta gcctcgagga tatcaagatc tggcctcggc
61 ggccaagctt ggcaatccgg tactgttggt aaagccacca tggtcttcac actcgaagat
121 ttcgttgggg actggcgaca gacagccggc tacaacctgg accaagtcct tgaacaggga
181 ggtgtgtcca gtttgtttca gaatctcggg gtgtccgtaa ctccgatcca aaggattgtc
241 ctgagcggtg aaaatgggct gaagatcgac atccatgtca tcatcccgta tgaaggtctg
301 agcggcgacc aaatgggcca gatcgaaaaa atttttaagg tggtgtaccc tgtggatgat
361 catcacttta aggtgatcct gcactatggc acactggtaa tcgacggggt tacgccgaac
421 atgatcgact atttcggacg gccgtatgaa ggcatcgccg tgttcgacgg caaaaagatc
481 actgtaacag ggaccctgtg gaacggcaac aaaattatcg acgagcgcct gatcaacccc
541 gacggctccc tgctgttccg agtaaccatc aacggagtga ccggctggcg gctgtgcgaa
601 cgcattctgg cgtaaggccg cgactctaga gtcggggcgg ccggccgctt cgagcagaca
661 tgataagata cattgatgag tttggacaaa ccacaactag aatgcagtga aaaaaatgct
721 ttatttgtga aatttgtgat gctattgctt tatttgtaac cattataagc tgcaataaac
781 aagttaacaa caacaattgc attcatttta tgtttcaggt tcagggggag gtgtgggagg
841 ttttttaaag caagtaaaac ctctacaaat gtggtaaaat cgataaggat ccgtttgcgt
901 attgggcgct cttccgctga tctgcgcagc accatggcct gaaataacct ctgaaagagg
961 aacttggtta gctaccttct gaggcggaaa gaaccagctg tggaatgtgt gtcagttagg
1021 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta
1081 gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat
1141 gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac
1201 tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga
1261 ggccgaggcc gcctctgcct ctgagctatt ccagaagtag tgaggaggct tttttggagg
1321 cctaggcttt tgcaaaaagc tcgattcttc tgacactagc gccaccatga agaagcccga
1381 actcaccgct accagcgttg aaaaatttct catcgagaag ttcgacagtg tgagcgacct
1441 gatgcagttg tcggagggcg aagagagccg agccttcagc ttcgatgtcg gcggacgcgg
1501 ctatgtactg cgggtgaata gctgcgctga tggcttctac aaagaccgct acgtgtaccg
1561 ccacttcgcc agcgctgcac tacccatccc cgaagtgttg gacatcggcg agttcagcga
1621 gagcctgaca tactgcatca gtagacgcgc ccaaggcgtt actctccaag acctccccga
1681 aacagagctg cctgctgtgt tacagcctgt cgccgaagct atggatgcta ttgccgccgc
1741 cgacctcagt caaaccagcg gcttcggccc attcgggccc caaggcatcg gccagtacac
1801 aacctggcgg gatttcattt gcgccattgc tgatccccat gtctaccact ggcagaccgt
1861 gatggacgac accgtgtccg ccagcgtagc tcaagccctg gacgaactga tgctgtgggc
1921 cgaagactgt cccgaggtgc gccacctcgt ccatgccgac ttcggcagca acaacgtcct
1981 gaccgacaac ggccgcatca ccgccgtaat cgactggtcc gaagctatgt tcggggacag
2041 tcagtacgag gtggccaaca tcttcttctg gcggccctgg ctggcttgca tggagcagca
2101 gactcgctac ttcgagcgcc ggcatcccga gctggccggc agccctcgtc tgcgagccta
2161 catgctgcgc atcggcctgg atcagctcta ccagagcctc gtggacggca acttcgacga
2221 tgctgcctgg gctcaaggcc gctgcgatgc catcgtccgc agcggggccg gcaccgtcgg
2281 tcgcacacaa atcgctcgcc ggagcgcagc cgtatggacc gacggctgcg tcgaggtgct
2341 ggccgacagc ggcaaccgcc ggcccagtac acgaccgcgc gctaaggagg taggtcgagt
2401 ttaaactcta gaaccggtca tggccgcaat aaaatatctt tattttcatt acatctgtgt
2461 gttggttttt tgtgtgttcg aactagatgc tgtcgaccga tgcccttgag agccttcaac
2521 ccagtcagct ccttccggtg ggcgcggggc atgactatcg tcgccgcact tatgactgtc
2581 ttctttatca tgcaactcgt aggacaggtg ccggcagcgc tcttccgctt cctcgctcac
2641 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
2701 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
2761 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
2821 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
2881 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
2941 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
3001 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
3061 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
3121 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
3181 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
3241 aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
3301 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
3361 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
3421 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
3481 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
3541 tgagtaaact tggtctgaca gcggccgcaa atgctaaacc actgcagtgg ttaccagtgc
3601 ttgatcagtg aggcaccgat ctcagcgatc tgcctatttc gttcgtccat agtggcctga
3661 ctccccgtcg tgtagatcac tacgattcgt gagggcttac catcaggccc cagcgcagca
3721 atgatgccgc gagagccgcg ttcaccggcc cccgatttgt cagcaatgaa ccagccagca
3781 gggagggccg agcgaagaag tggtcctgct actttgtccg cctccatcca gtctatgagc
3841 tgctgtcgtg atgctagagt aagaagttcg ccagtgagta gtttccgaag agttgtggcc
3901 attgctactg gcatcgtggt atcacgctcg tcgttcggta tggcttcgtt caactctggt
3961 tcccagcggt caagccgggt cacatgatca cccatattat gaagaaatgc agtcagctcc
4021 ttagggcctc cgatcgttgt cagaagtaag ttggccgcgg tgttgtcgct catggtaatg
4081 gcagcactac acaattctct taccgtcatg ccatccgtaa gatgcttttc cgtgaccggc
4141 gagtactcaa ccaagtcgtt ttgtgagtag tgtatacggc gaccaagctg ctcttgcccg
4201 gcgtctatac gggacaacac cgcgccacat agcagtactt tgaaagtgct catcatcggg
4261 aatcgttctt cggggcggaa agactcaagg atcttgccgc tattgagatc cagttcgata
4321 tagcccactc ttgcacccag ttgatcttca gcatctttta ctttcaccag cgtttcgggg
4381 tgtgcaaaaa caggcaagca aaatgccgca aagaagggaa tgagtgcgac acgaaaatgt
4441 tggatgctca tactcgtcct ttttcaatat tattgaagca tttatcaggg ttactagtac
4501 gtctctcaag gataagtaag taatattaag gtacgggagg tattggacag gccgcaataa
4561 aatatcttta ttttcattac atctgtgtgt tggttttttg tgtgaatcga tagtactaac
4621 atacgctctc catcaaaaca aaacgaaaca aaacaaacta gcaaaatagg ctgtccccag
4681 tgcaagtgca ggtgccagaa catttctct
//
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